| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031
|
| Autores: |
Garnier, Matthieu
Matamoros, Sebastien
Chevret, Didier
Pilet, Marie-france
Leroi, Francoise
Tresse, Odile
|
| Data: |
2010-12
|
| Ano: |
2010
|
| Resumo: |
There is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. The psychrotrophic biopreservative Lactococcus piscium strain CNCM I-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. Growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described before for lactic acid bacteria (LAB). A comparative proteomic analysis was applied with two-dimensional gel electrophoresis (2-DE), and upregulated proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both cold shock and cold acclimation triggered the upregulation of proteins involved in general and oxidative stress responses and fatty acid and energetic metabolism. However, 2-DE profiles and upregulated proteins were different under both conditions, suggesting a sequence of steps in cold adaptation. In addition, the major 7-kDa Csp protein was identified in the L. piscium CNCM I-4031 genome but was not cold regulated. The implication of the identified cold shock proteins and cold acclimation proteins in efficient cold adaptation, the possible regulation of a histidyl phosphocarrier protein, and the roles of a constitutive major 7-kDa Csp are discussed.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00019/13047/10150.pdf
DOI:10.1128/AEM.01331-10
|
| Editor: |
Amer Soc Microbiology
|
| Relação: |
http://archimer.ifremer.fr/doc/00019/13047/
|
| Formato: |
application/pdf
|
| Fonte: |
Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2010-12 , Vol. 76 , N. 24 , P. 8011-8018
|
| Direitos: |
2010 American Society for Microbiology. All Rights Reserved.
|
|
